Different patterns of HIV-1 DNA after therapy discontinuation

BACKGROUND: By persisting in infected cells for a long period of time, proviral HIV-1 DNA can represent an alternative viral marker to RNA viral load during the follow-up of HIV-1 infected individuals. In the present study sequential blood samples of 10 patients under antiretroviral treatment from 1997 with two NRTIs, who refused to continue any antiviral regimen, were analyzed for 16 - 24 weeks to study the possible relationship between DNA and RNA viral load. METHODS: The amount of proviral DNA was quantified by SYBR green real-time PCR in peripheral blood mononuclear cells from a selected group of ten patients with different levels of plasmatic viremia (RNA viral load). RESULTS: Variable levels of proviral DNA were found without any significant correlation between proviral load and plasma HIV-1 RNA levels. Results obtained showed an increase or a rebound in viral DNA in most patients, suggesting that the absence of therapy reflects an increase and/or a persistence of cells containing viral DNA. CONCLUSION: Even though plasma HIV RNA levels remain the basic parameter to monitor the intensity of viral replication, the results obtained seem to indicate that DNA levels could represent an adjunct prognostic marker in monitoring HIV-1 infected subjects. Key words: HIV-1DNA viral load, therapy interruption

PI and OPG/RANKL levels in human osteoblast cells

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Circulating and Tumor-Associated Neutrophils in the Era of Immune Checkpoint Inhibitors: Dynamics, Phenotypes, Metabolism, and Functions.

Neutrophils are the most abundant myeloid cells in the blood and are a considerable immunological component of the tumor microenvironment. However, their functional importance has often been ignored, as they have always been considered a mono-dimensional population of terminally differentiated, short-living cells. During the last decade, the use of cutting-edge, single-cell technologies has revolutionized the classical view of these cells, unmasking their phenotypic and functional heterogeneity. In this review, we summarize the emerging concepts in the field of neutrophils in cancer, by reviewing the recent literature on the heterogeneity of both circulating neutrophils and tumor-associated neutrophils, as well as their possible significance in tumor prognosis and resistance to immune checkpoint inhibitors

The challenge of sharing data in cooperation projects: cause for reflection.

The collection of data and  their management  remain a never-ending challenge within projects carried out in low-income countries.  Data collection requires high investments, and even if great efforts have been done in building tools for data sharing, these processes need to be improved at local level in developing countries. Data are valuable items  for developing strategies leading to more effective local development. The CeTAmb LAB, on the basis of its experience, proposes a reflection on the role of the academia, which is based on principles of capacity building and sharing knowledge, in promoting the importance of data collection, conservation and sharing

Increased plasma levels of mitochondrial DNA and pro-inflammatory cytokines in patients with progressive multiple sclerosis

The role of damage-associated molecular patterns in multiple sclerosis (MS) is under investigation. Here, we studied the contribution of circulating high mobility group box protein 1 (HMGB1) and mitochondrial DNA (mtDNA) to neuroinflammation in progressive MS. We measured plasmatic mtDNA, HMGB1 and pro-inflammatory cytokines in 38 secondary progressive (SP) patients, 35 primary progressive (PP) patients and 42 controls. Free mtDNA was higher in SP than PP. Pro-inflammatory cytokines were increased in progressive patients. In PP, tumor necrosis factor-α correlated with MS Severity Score. Thus, in progressive patients, plasmatic mtDNA and pro-inflammatory cytokines likely contribute to the systemic inflammatory status

Del espacio virtual al espacio social: hacia una nueva metodología de la pintura mural aplicada a la rehabilitación del espacio urbano

La continua evolución de técnicas y soportes ha llevado a abordar la docencia artística contemporánea no sólo desde desde la maestría de un material concreto, como la cal, el óleo o el temple, sino también específicamente desde áreas abstractas de investigación. Así observamos en la última actualización del programa de la asignatura Pintura Mural un esfuerzo por integrar las problemáticas “que aparecen en la interacción entre los espacios y expresiones pictórica y arquitectónica”. Sin embargo, es necesario remarcar que la Pintura Mural en el momento actual se ha expandido hacia la intervención artística en espacios y contextos muy heterogéneos, de tal manera que la práctica artística queda unida al contexto social y urbanístico en el cual se desarrolla, ejerciendo una importante labor dinamizadora El presente proyecto de Innovación Tecnológica surge de la necesidad de fomentar pensamiento crítico en torno a la repercusión social de las intervenciones realizadas en un espacio/arquitectura determinado y de dotar al alumno de herramientas contemporáneas de análisis y seguimiento de tal repercusión, aportando al actual programa la incorporación de las nuevas tecnologías en las fases de anteproyecto, proyecto y producción de las intervenciones y el apoyo tecnológico a la docencia presencial y semipresencial. El proyecto plantea el desarrollo de una herramienta web on-line, que será transferible, open source, susceptible de ser utilizada en cualquier entorno social en el que se quieran realizar intervenciones urbanas, y por tanto podrá ser empleada por: ayuntamientos, juntas de distritos, centros de integración social, espacios institucionales, facultades de Bellas Artes, Sociología, etc. La herramienta, concebida como un contenedor de información (localizaciones y cartografías digitales, mapas de controversias, documentación fotográfica, brain storms, links de interés contextual, etc.), funciona como aula virtual

Human T-lymphotropic virus type 1 (HTLV-1) prevalence and quantitative detection of DNA proviral load in individuals with indeterminate/positive serological results

BACKGROUND: HTLV-1 infection is currently restricted to endemic areas. To define the prevalence of HTLV-1 infection in patients living in Italy, we first carried out a retrospective serological analysis in a group of people originating from African countries referred to our hospital from January 2003 to February 2005. We subsequently applied a real time PCR on peripheral blood mononuclear cells from subjects with positive or indeterminate serological results. METHODS: All the sera were first analysed by serological methods (ELISA and/or Western Blotting) and then the peripheral blood mononuclear cells from subjects with positive or inconclusive serological results were analyzed for the presence of proviral DNA by a sensitive SYBR Green real time PCR. In addition, twenty HTLV-I ELISA negative samples were assayed by real time PCR approach as negative controls. RESULTS: Serological results disclosed serum reactivity by ELISA (absorbance values equal or greater than the cut-off value) in 9 out of 3408 individuals attending the Sexually Transmitted Diseases Clinic and/or Oncology Department, and 2 out 534 blood donors enrolled as a control population. Irrespective of positive or inconclusive serological results, all these subjects were analyzed for the presence of proviral DNA in peripheral blood mononuclear cells by SYBR real time PCR. A clear-cut positive result for the presence of HTLV-1 DNA was obtained in two subjects from endemic areas. CONCLUSION: SYBR real time PCR cut short inconclusive serological results. This rapid and inexpensive assay showed an excellent linear dynamic range, specificity and reproducibility readily revealing and quantifying the presence of virus in PBMCs. Our results highlight the need to monitor the presence of HTLV-1 in countries which have seen a large influx of immigrants in recent years. Epidemiological surveillance and correct diagnosis are recommended to verify the prevalence and incidence of a new undesirable phenomenon